Below we list the basic study items by field. If you are interested in a study other than those listed below, please check our track record of custom-made studies. If you would like to undergo reliability standard application study for pharmaceutical applications, etc., please check our reliability assurance system here. We also welcome direct inquiries via the contact form.
Various study items (Click on each study name to see the study summary and links to study details.)
Immunology and inflammation-related Studies
pDC activation study
Using highly purified plasmacytoid dendritic cells (pDCs) induced by differentiation, we will identify and evaluate their activating substances. >>Study details
Immune function regulation study
Using macrophages, which play a central role in immune responses, we evaluate the cytokine production effects of the test substance. >>Study details
Inflammation suppression study
We evaluate the anti-inflammatory effects of test substances on various cell types, including human immune cells. >>Study details
Study on suppression of atopic dermatitis
An atopic dermatitis model will be created using human epidermal keratinocytes, and the effect of test substances on improving atopic dermatitis will be evaluated by various gene expression analyses. >>Study details
Study on inhibition of histamine release
The histamine-releasing effect of the test substance is evaluated by measuring the amount of free histamine using rat mast cells. >>Study details
Study on promotion of IgA production
By evaluating IgA production using mouse Peyer’s patch cells, we will search for food ingredients that enhance IgA production in the intestinal tract by the test substance and evaluate immune adjuvants. >>Study details
Evaluation of Anti-Coronavirus Activity
The anti-coronavirus effects of test substances will be evaluated using a pseudovirus containing the SARS-CoV2 S protein and ACE2-expressing cells. >>Study details
Study on suppression of enteritis
Intestinal epithelial cells are used to evaluate the anti-inflammatory effect of the test substance on intestinal tissue. >>Study details
Study on pulmonary function improvement
Using human lung cells, the anti-inflammatory effect of test substances is evaluated using cytokine production as an indicator. >>Study details
Cosmetic-related studies
Melanin production suppression study (plate culture)
The melanin production inhibitory effect of the test substance is evaluated using mouse-derived B16 melanoma cells and human melanocytes. >>Study details
Melanin production suppression study (3D model)
The melanin production inhibitory effect of test substances is evaluated using a melanocyte-containing human three-dimensional skin model that is more similar to in vivo. >>Study details
Fibroblast activation study
Using human skin fibroblasts, we will evaluate functional substances that bring firmness and moisture to the skin by measuring the production of type I collagen and hyaluronic acid. >>Study details
UV protection study
Using human epidermal keratinocytes, the test substance’s ability to protect and avoid stress from UV rays is evaluated. >>Study details
Study on promotion of moisturization and barrier function
Using human epidermal keratinocytes, we evaluate the moisturizing and barrier function-promoting effects of test substances through gene expression analysis related to ceramide and natural moisturizing factor synthesis. >>Study details
Dry protection study
By exposing human epidermal keratinocytes and corneal cells to dry conditions, the effectiveness of test substances in improving dry skin and dry eyes will be evaluated. >>Study details
Promotion of cell migration/wound healing
Using human skin-related cells, the wound healing effects of test substances are evaluated by measuring the proliferation and migration ability of the cells through imaging analysis. >>Study details
Dermal papilla cell activation
Using human dermal papilla cells, the hair growth and hair regrowth effects of the test substance are evaluated by measuring cell proliferation rates and the production of hair growth promoting factors FGF-7 and VEGF. >>Study details
Male Pattern Hair Loss (AGA) suppression study
The inhibitory effect of the test substance on hair loss factors is evaluated by measuring the amount of hair loss factors produced using human dermal papilla cells. >>Study details
Metabolism-related studies
Metabolic function evaluation using a flux analyzer
Using an extracellular flux analyzer, we evaluate the cellular energy metabolic state in various cell lines in real time. >>Study details
Study on suppression of adipocyte differentiation
Using mouse adipocyte cell lines and human preadipocytes, we will evaluate the anti-obesity effects of test substances by inhibiting differentiation into adipocytes. >>Study details
Lipolysis promotion study
Using mouse adipocyte cell lines and differentiated adipocytes derived from human preadipocytes, the anti-obesity effect of test substances by promoting lipolysis is evaluated by quantifying free glycerol. >>Study details
Adipose function improvement study
We will evaluate the effect of improving fat function by converting white fat cells, which store energy in the form of neutral fat, to brown fat cells, which consume energy. >>Study details
Mitochondrial activation evaluation
Using various cell types, we measure intracellular mitochondrial activity and mitochondrial quantity to evaluate the mitochondrial activation effect of the test substance. >>Study details
Glucose uptake assessment
Using adipocytes and skeletal muscle cells, we will evaluate the anti-diabetic effect by promoting glucose uptake and the anti-obesity effect by inhibiting glucose uptake. >>Study details
Study on improvement of bone metabolism
We evaluate the bone formation and metabolic functions of test substances by examining their effects on differentiation into osteoblasts and osteoclasts using various bone-related cells. >>Study details
Cartilage function improvement
Using rabbit chondrocytes, etc., the amount of hyaluronic acid and acidic mucopolysaccharides produced is measured to evaluate the cartilage function-improving effect of the test substance. >>Study details
Muscle enhancement study
Using mouse myoblast cell lines and human myoblast cells, the myotube differentiation-inducing effect of test substances is evaluated by imaging analysis. >>Study details
Muscle atrophy suppression study
Myotubes are formed from mouse myoblasts and other cells, and the muscle atrophy inhibitory effect of the test substance is evaluated by quantifying the expression of proteolytic enzymes under muscle atrophy induction stimuli. >>Study details
Liver function improvement study
Using liver cancer-derived cell lines and primary cultured human hepatocytes, we will analyze the gene expression of various metabolic enzymes to evaluate the liver function-improving effects of test substances. >>Study details
Autophagy promotion study
Using various cells, the autophagy-promoting effect of test substances is evaluated by quantifying the number of autophagic vesicles through imaging analysis. >>Study details
Aging-related studies
Study on removal of senescent cells
Using normal human fibroblast cell lines and passage-aged fibroblasts, the selective elimination of senescent cells by the test substance will be evaluated. >>Study details
By introducing a microscope culture system, we are able to perform real-time ecological observations under a microscope. >>Study and Equipment introduction
Blood vessel and blood-related studies
Study on promotion/inhibition of angiogenesis
Using human vascular endothelial cells, etc., the angiogenic effects of test substances are evaluated using high-content screening equipment. >>Study details
Vascular function improvement study
Using vascular endothelial cells, we will verify the effect of the test substance on improving vascular function by measuring factors with vasodilatory and antithrombotic effects. >>Study details
Evaluation of anti-atherosclerotic effects
An arteriosclerosis model will be created using human aortic smooth muscle cells, and the anti-atherosclerotic effects of the test substance will be evaluated. >>Study details
Study on promotion of blood cell differentiation
Using human myeloid leukemia cells (HL-60) and other cells, the ability of the test substance to promote differentiation into various blood cells is evaluated. >>Study details
Neurological related studies
Evaluation of neurite outgrowth promotion
Using neuronal cell lines, we will evaluate neurite outgrowth-promoting activity and stress protection against amyloid beta through imaging analysis. >>Study details
Study on promotion of NGF production
By measuring neurotrophic factors, which are important for neuronal differentiation, regeneration, and maintenance, using glial cells, we evaluate the neurotrophic factor production-promoting effect of test substances. >>Study details
Cancer-related studies
Cell proliferation inhibition study
We screen and evaluate novel compounds with anticancer activity by measuring cell proliferation rates using various cancer cell lines. >>Study details
Apoptosis induction study
Using various cell lines, including cancer cell lines, we evaluate the effect of test substances on apoptosis induction through imaging analysis. >>Study details
Study on inhibition of cancer cell invasion
Using a basement membrane model, the inhibitory effect of test substances on cancer cell invasion is evaluated. >>Study details
Biochemical studies and Molecular biology studies
Protein expression analysis using Simple Western
Protein Simple’s fully automated Western blotting system is used to perform quantitative analysis of protein expression. >>Study and Equipment introduction
Proteome analysis (in collaboration with partner companies)
We provide RNA extraction from cells cultured under your desired cell type and conditions, DNA microarray analysis, and next-generation sequencing analysis. >>Study details
Gene forced expression and overexpression evaluation
We can produce transiently expressing cells or stable expressing cells according to your needs and then perform various evaluation studies using those cells. >>Study details
siRNA knockdown evaluation
We use various cells to perform gene knockdown and various evaluation studies using those cells. >>Study details
Tyrosinase activity inhibition
The inhibitory effect of test substances on tyrosinase activity is evaluated using a cell-free in vitro assay. >>Study details
Collagenase activity inhibition
A cell-free in vitro assay is used to evaluate the inhibitory effect of test substances on collagenase activity. >>Study details
NADPH oxidase activity measurement
The NADPH oxidase activity of test substances is evaluated using a cell-free in vitro assay. >>Study details
Potency evaluation studies
Recombinant protein evaluation
We will support you from proposing the cells and test systems suitable for evaluation of your target recombinant protein to activity evaluation. >>Study details
Substrate evaluation studies
Substrate evaluation
Using the substrate you provide, we will respond to your needs by proposing the cells and assay system suitable for evaluation, evaluating the substrate, and providing feedback on its use. >>Study details
iPS cell-related studies
iPS cell-based study
We support various experiments, research, and development related to iPS cells. >>Study details
Signal Molecule Interaction Evaluation study
Various reporter assay
We can handle various tests using cells for your specified reporter assay. Please contact us directly for details. >>Contact us
ChIP-seq
We can handle tests and analyses according to your needs, from sample preparation from cells to ChIP, data analysis and interpretation, etc. >>Study details
Others
Establishment and storage of primary culture cells
We can accommodate your needs, from establishing primary cultured cells to investigating special culture methods such as three-dimensional culture and serum-free culture. >>Study details
