Study Overview
Lipolysis is the biological process where neutral fats (triglycerides) stored within fat cells (adipocytes) are broken down into fatty acids and glycerol. This metabolic pathway is primarily triggered when the body requires extra energy, such as during fasting, exercise, or exposure to cold. Substances that are capable of inducing or promoting lipolysis are sought after as anti-obesity drugs.
This assay is intended to evaluate functional substances that are to induce lipolysis, thereby inducing weight loss. Substances will be tested in adipocytes that have already undergone differentiation. To determine the extent of lipolysis, glycerol is measured as a readout.
Study example
Basic Experimental design
| Cells used | Mouse adipocytes (3T3-L1) Human preadipocytes (subcutaneous and visceral) |
|---|---|
| Culture period | Samples were treated for 24 hours on adipocytes on day 11 of differentiation induction. |
| Positive control | Isoproterenol |
| Measurement method | Free glycerol measurement (measured by absorbance) |
*All our studies are mainly performed and handled by our team of PhD scientists.
