Study Overview
An appropriate amount of muscle is maintained through a balance between muscle synthesis through differentiation from myoblasts and muscle degradation via the ubiquitin-proteasome system. When this balance is disrupted by aging or various diseases, and muscle degradation becomes dominant, muscle atrophy occurs, resulting in a loss of muscle mass. Because a loss of muscle mass has a significant impact on daily life, measures to prevent muscle atrophy are also important from the perspective of quality of life.
This study evaluates the muscle atrophy inhibitory effect of the test substance primarily by quantifying the expression of proteolytic enzymes. Custom-made studies are also available to elucidate the detailed mechanisms.
Study example
Basic Experimental design
| Cells used | Mouse myoblast cell line C2C12 (Can also be used with L6 cells, normal human myoblasts, etc.) |
|---|---|
| Positive control | Insulin |
| Preliminary study | Cytotoxicity assay |
| Main study | Expression analysis of protein degradation-related genes (Atrogin-1, Murf-1) |
*Studies will be mainly conducted by those with doctoral degrees.
