Study Overview
Muscle mass is maintained by balancing synthesis from myoblasts with degradation via the ubiquitin-proteasome system. When aging or disease disrupts this balance and degradation dominates, muscle atrophy occurs. Preventing this loss of mass is essential for maintaining quality of life and daily function, especially during the aging process.
This assay evaluates test substances for their capability to inhibit muscle atrophy. This is mainly quantified by the measurement of proteolytic enzymes. Customized studies are also available to understand the mechanism of action of atrophy inhibition.
Study example
Basic Experimental design
| Cells used | Mouse myoblast cell line C2C12 (Can also be used with L6 cells, normal human myoblasts, etc.) |
|---|---|
| Positive control | Insulin |
| Preliminary study | Cytotoxicity assay |
| Main study | Expression analysis of protein degradation-related genes (Atrogin-1, Murf-1) |
*All our studies are mainly performed and handled by our team of PhD scientists.
