Study Overview
Skin and eyeballs are tissues constantly exposed to dryness stress, but epidermal keratinocytes in skin and corneal cells in eyeballs are the outermost tissues, and they protect the tissue from internal dryness by forming strong intercellular bonds (tight junctions). Therefore, the state of tight junctions is an important indicator for evaluating the tissue’s protective function against dryness.
In this study, we will stain occludin, one of the key proteins that form tight junctions, to evaluate the state of tight junctions in cells after exposure to dryness stress. This will enable us to verify whether the test substance is effective in improving dry skin and dry eye.
Occludin antibody staining in immortalized human corneal epithelial cells
Basic Experimental design
| Cells used | Immortalized human corneal epithelial cells, human epidermal keratinocytes, etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Live cell count (WST-8 method) Occludin antibody staining |
*Studies will be mainly conducted by those with doctoral degrees.
