Study Overview
The skin and eyes of our bodies are always exposed to the environment, losing moisture constantly. The cells in the eyes and skin have specialized functions adapted to such conditions, protecting the inner cells from drying out. By forming tight connections between cells, a strong barrier (called “tight junctions”) that retains moisture is formed. As such, research into the conditions of tight junctions can provide important insight into how the tissue protects itself from dryness.
In this study, we measured the effects of a test substance on occludin. Occludin is a protein that helps cells form and maintain these tight junctions, preserving moisture. By applying a stressor in the form of dryness in these cells, we evaluated the effect of a test substance in rescuing the phenotypes associated with dryness.
Occludin antibody staining in immortalized human corneal epithelial cells
Basic Experimental design
| Cells used | Immortalized human corneal epithelial cells, human epidermal keratinocytes, etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Live cell count (WST-8 method) Occludin antibody staining |
*All our studies are mainly performed and handled by our team of PhD scientists.
