Study Overview
Autophagy is a process in which cells themselves break down unnecessary proteins and recycle them into new proteins, thereby maintaining a healthy intracellular environment. Autophagy has been reported to be deeply involved in aging, cancer, and the development of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease, and is attracting a great deal of attention.
This study will examine the autophagy-promoting effect of the test substance.
Study example
We have introduced Operetta CLS and Opera Phenix Plus, high-content screening instruments that enable high-speed cell-based imaging analysis, allowing us to analyze the autophagy activity state of living cells with high precision and high throughput.
Basic Experimental design
| Cells used | Various cells such as liver cells and nerve cells |
|---|---|
| Measurement items | Autophagy fluorescent staining and quantification |
References
●Longevity and autophagy
The role of autophagy in aging: its essential part in the anti-aging mechanism of caloric restriction
Bergamini E, Cavallini G, Donati A, Gori Z., Ann N Y Acad Sci. 2007
●Age-related decline in autophagy
Autophagic activity in thymus and liver during aging
Uddin MN, Nishio N, Ito S, Suzuki H, Isobe K., Age. 2012
●Cancer and autophagy
Role of autophagy in cancer
Mathew R, Karantza-Wadsworth V, White E., Nat Rev Cancer. 2007
●Alzheimer disease and autophagy
Extensive involvement of autophagy in Alzheimer disease: an immuno-electron microscopy study
Nixon RA, Wegiel J, Kumar A, Yu WH, Peterhoff C, Cataldo A, Cuervo AM., J Neuropathol Exp Neurol. 2005
●Parkinson’s disease and autophagy
NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy
Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N., EMBO Rep. 2018
*Studies will be mainly conducted by those with doctoral degrees.
