Study Overview
Bone may appear stationary, but it is constantly undergoing metabolism.
Bone is formed by osteoblasts.
Bone is broken down by osteoclasts.
This test examines how your sample effects osteoblasts and osteoclasts.
This reveals whether it strengthens or weakens bone.
Mouse osteoblastic cell line (MC3T3-E1)
Osteoclasts differentiated from rat bone marrow cells
(TRAP staining image)
Study example
Basic Experimental design
| Cells used | Primary osteoblasts, MC3T3-E1, etc. Primary osteoclast precursor cells, Raw264.7, etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | <Studies using osteoblasts> ・Cell activation study ・ALP activity measurement ・Osteocalcin level measurement ・Alizarin red staining, etc. <Studies using osteoclasts> ・Cell activation test ・TRAP staining, etc. |
*Studies will be mainly conducted by those with doctoral degrees.
