Study Overview
Ceramides and natural moisturizing factors (NMFs) are known to be important factors for skin moisturization and barrier function. Ceramides are intercellular lipids that fill the gaps between stratum corneum cells and function to firmly connect cells together. NMFs are moisturizing components produced from filaggrin. These substances enable skin to retain sufficient moisture and protect tissues from damage caused by external stimuli. However, it is known that the production of these substances decreases with aging, resulting in a decline in moisturizing and barrier function. As such, substances that are able to promote ceramides and NMFs in skin cells are of interest to the cosmetic industry to improve skin health.
This study evaluates the effects of test substances that are able to promote the synthesis of ceramides and NMFs, improving skin moisturization and skin barrier function.
Basic Experimental design
| Cells used | Normal human epidermal keratinocyte |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of filaggrin (FLG) and serine palmitoyltransferase (SPT) gene expression levels (Analysis by real-time PCR） |
*All our studies are mainly performed and handled by our team of PhD scientists.
