Study Overview
Ceramides and natural moisturizing factors (NMFs) are known to be important factors for skin moisturization and barrier function. Ceramides are intercellular lipids that fill the gaps between stratum corneum cells and function to firmly connect cells together. NMFs are moisturizing components produced from filaggrin. These substances enable skin to retain sufficient moisture and protect tissues from damage caused by external stimuli. However, it is known that the production of these substances decreases with aging, resulting in a decline in moisturizing and barrier function.
This study will verify the moisturizing and barrier function-promoting effects of test substances by measuring the expression levels of genes involved in the synthesis of ceramides and NMFs.
Basic Experimental design
| Cells used | Normal human epidermal keratinocyte |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of filaggrin (FLG) and serine palmitoyltransferase (SPT) gene expression levels (Analysis by real-time PCR） |
*Studies will be mainly conducted by those with doctoral degrees.
