Products

• Product Information
• Product Features
• How to Purchase
• Frequently asked questions
• Reference

Product Information

Product Document Download

• Human Collagen Type I ELISA (without pepsin) EC1-E105 PDF
• Human Collagen Type I ELISA (with pepsin) EC1-E205 PDF
• Pepsin for Human collagen Type I ELISA kit EC1-E110 PDF

Product Features

• Native Collagen Measurement
  This ELISA kit allows for the quantification of collagen that maintains its natural molecular structure. (For amino acid analysis, the molecular structure is broken down and the amount of hydroxyproline is measured.)
• Simple
  The system uses a 96-well microtiter plate, allowing for simultaneous measurement of multiple samples.
  (Up to 80 samples in single replicates, 40 samples in duplicates, and 26 samples in triplicates.)
• Rapid
  Collagen is pre-immobilized on the plate, allowing for rapid quantification (reaction time of 2 hours and 30 minutes).

Measurement procedure

Cross-reactivity

Cross-reactivity to other species and collagen types was compared to reactivity to human collagen type I (100%)

Standard curve

The collagen concentration in the sample was calculated based on the standard curve
(calculated using a 4-parameter logistic curve)

How to Purchase

It can be purchased from Funakoshi Co., Ltd., Cosmo Bio Co., Ltd. or Inmedium, Inc.

Product name	Capacity	Product Code	Where to buy
Human Collagen Type I ELISA (with pepsin)	1 kit	EC1-E105
Human Collagen Type I ELISA (without pepsin)	1 kit	EC1-E205
Pepsin for Human collagen Type Ⅰ ELISA kit	500 mg	EC1-E110

Frequently asked questions

I would like to evaluate changes in collagen levels in cultured cells. Is this possible?

It is possible to measure collagen both in the culture supernatant and deposited as extracellular matrix.

Does the serum concentration in the culture medium affect the measurement?

There is no limit to serum concentration.

Please tell me the range of measurable concentrations.

The detection limits are 0.02 µg/mL at the lower end and 40 µg/mL at the upper end.
Since standards of 0.16 to 10 µg/mL are used in actual measurements, this range is recommended.

Can samples derived from animals other than humans be measured?

It cannot be measured because the antibody does not react.

What antibodies are being used?

We use a polyclonal antibody that uses atelocollagen as an antigen.
Because atelocollagen is used as an antigen, we believe it will recognize the triple helix.

What is the pH of the “200 mM Tris, 150 mM NaCl solution” used in the measurement?

No pH adjustment is necessary. For example, the pH of a “200mM Tris, 150mM NaCl solution” prepared using Tris and NaCl from Fujifilm Wako Pure Chemical Industries, Ltd. is approximately 10.5.

I’m planning to purchase a plate reader. Could you tell me the measurement wavelength?

The measurement wavelength is 450 nm.

Regarding collagen measurement in culture supernatant, please tell us which cell lines have been used for measurement.

We have experience with human dermal fibroblasts and human lung fibroblasts.

References

*If you use this product in a paper, please let us know along with a copy of the paper. We will send you a complimentary gift.

In Vitro CO-Releasing and antioxidant properties of sulfonamide-based CAI-CORMs in a H2O2-stimulated human achilles tendon-derived cell model
Molecules 2025
 
Silencing of ERRα gene represses cell proliferation and induces apoptosis in human skin fibroblasts.
Molecular Medicine Reports 2024
 
Snail slime extracted by a cruelty free method preserves viability and controls inflammation occurrence: a focus on fibroblasts.
Molecules 2023
 
The open cell form of 3D-printed titanium improves osteconductive properties and adhesion behavior of dental pulp stem cells.
Materials 2021
 
Liposomes embedded with differentiating factors as a new strategy for enhancing DPSC osteogenic commitment.
European Cells & Materials Journal 2021
 
Dual acting carbon monoxide releasing molecules and carbonic anhydrase inhibitors differentially modulate inflammation in human tenocytes.
Biomedicines 2021
 
Bisphosfonate matrix metalloproteinase inhibitors for the treatment of periodontitis: An in vitro study.
International Journal of Molecular Medicine 2018
 
Intestinal myofibroblast TRPC6 channel may contribute to stenotic fibrosis in crohn’s disease.
Inflammatory Bowel Diseases 2015
 
Differences between protein expression and extracellular matrix state on uniaxial stretching for tenogenic differentiation.
Journal of Mechanics in Medicine and Biology 2014
 
Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy.
British Journal of Dermatology 2011