Study Overview
Bacterial pneumonia is the most common form of pneumonia caused by the invasion of bacteria into the pulmonary alveoli of the lungs. The symptoms of pneumonia are caused by the production of inflammatory cytokines in response to the bacteria, causing cellular damage and respiratory distress. By inhibiting the production of cytokines and reducing inflammation, symptoms of pneumonia can be potentially alleviated.
In this study, lung cells such as A549 cells are used to evaluate the anti-inflammatory effects of a test substance.
Study details
LPS was used to induce inflammatory cytokine production in A549 cells to evaluate the anti-inflammatory effects of a test substance. Cytokine production was measured as a readout of anti-inflammatory activity using this system. Both test substances B and C were determined to have an inhibitory effect on inflammatory cytokine production in this study.
Basic Experimental design
| Cells used | Human lung adenocarcinoma-derived cells (A549), etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Live cell count measurement, inflammatory cytokine measurement (after LPS addition) |
*All our studies are mainly performed and handled by our team of PhD scientists.
