Study Overview
During muscle differentiation, undifferentiated myoblasts fuse to form multinucleated myotubes, which then mature into contractile muscle fibers. Maintaining muscle mass is essential for anti-aging, as it naturally declines with age. Identifying substances that are able to promote muscle mass increase or maintain muscle mass are important for anti-aging or for recovery of muscle. This assay evaluates if a substance is capable of promoting muscle mass increase and maintenance of muscle mass by measuring Myosin heavy chain (MHC), Multinucleation and myotube differentiation rates.
Study example
Basic Experimental design
| Cells used | Mouse myoblast cell lines C2C12, L6 cells, normal human myoblasts, etc. |
|---|---|
| Measurement items | Myosin heavy chain (MHC) staining Multinucleation quantification Differentiation rate measurement of myotubes using quantitative imaging analysis. |
*All our studies are mainly performed and handled by our team of PhD scientists.
