Study Overview
Since many of the studies we perform involve the use and production of cell lines, we have developed the capabilities to establish and store a wide range of cells. Many of these cell lines require specialized culture methods and conditions, such as three-dimensional or serum-free cultures. Over many experiments, we have developed extensive protocols for establishing and storing a wide variety of cell lines with specialized needs.
Establishment of primary cell cultures
Primary culture example
- PBMC
- Peyer’s patch
- Spleen cells
- Various neurons (e.g, astrocytes, glial cells, oligodendrocytes)
- Preadipocytes
- Myoblast
- Vascular endothelial cells, etc.
Cell and product preparation
- High density culture (adherent cell culture)
- Preparation of cells and culture supernatant
Evaluation studies using special cultures and culture systems
- High density culture (adherent cell culture)
- Serum-free culture, Hollow fiber culture, Custom-made culture, etc.
- Spheroid cultures
- Conversion of adherent cells to suspension cells
Cell Storage (Cell Culture Option)
- Storage in liquid nitrogen (1×106 cells/ml・1ml)
Important Notes Regarding Sample Acceptance
① We will only handle samples that meet the following criteria and are non-infectious and non-toxic:
- Human-derived cells: Confirmed to be negative for HIV, HAV, HBV, HCV, and Treponema pallidum
- Animal-derived cells: Confirmed to be free of viruses, bacteria, etc. infectious to humans
② Please note that the establishment of primary cell cultures is limited to research purposes and we do not provide services for clinical use.
*When submitting samples to us as part of your study, please provide the accompanying documents such as CoA, testing records, source of cells etc. indicating the cells are free from pathogens.
*All our studies are mainly performed and handled by our team of PhD scientists.
