Study Overview
High content screening (HCS), sometimes referred to as high content analysis (HCA), is an analytical method that combines the advantages of fluorescence microscopy, flow cytometry, and high-throughput screening. It enables high-speed cell-based imaging analysis, enabling a consistent process from cell imaging to quantitative analysis of image data and presentation of analytical results. It is particularly effective for analyzing adherent cells.
We conduct cell-based screening assays to evaluate compounds, antibody drugs, nucleic acid drugs, and other compounds in the drug discovery field. We also undertake screening assays aimed at discovering new ingredients for cosmetics and functional foods. It can also be used in the field of regenerative medicine.
Our company uses the Operetta CLS and Opera Phenix Plus (manufactured by Revvity) as HCS instruments. The Operetta CLS and Opera Phenix Plus boast world-class market shares as HCS instruments capable of high speed, high resolution, and versatile applications. We use these instruments to perform custom-made tests using various cell types.
Please feel free to contact us.
Equipment used
Operetta CLS (Revvity)
Opera Phenix Plus (Revvity)
Features of the high content analysis
- Obtain a variety of quantitative data from individual cells
- Quantify cellular characteristics such as morphology and localization
- High-speed processing of large samples
- Analyze adherent cells and 3D structures directly
- Integrate measurement and analysis
- Various analytical applications
- Advanced analysis
Image analysis application examples
We can also customize your analysis and reproduce protocols used with other imaging equipment.
Please feel free to contact us.
Accurate cell recognition
Operetta CLS and Opera Phenix Plus can automatically recognize cells and other objects with extremely high accuracy from image data.
Apoptosis analysis example
Apoptosis was induced in HeLa cells by treating them with staurosporine. After four hours of treatment with the drug, the cells were fixed and nuclei were stained with DRAQ5 (red) and Alexa488-conjugated anti-Caspase 3 antibody (green) to detect caspase-3, a regulator of apoptosis. It can be seen that caspase-3 activation (left graph), nuclear aggregation, and nuclear fragmentation (right graph) occur in a concentration-dependent manner. This allows simultaneous analysis of multiple parameters, such as caspase-3 activity, nuclear size, and fragmentation rate, for samples at multiple concentrations.
Example of neurite analysis
Rat primary dorsal root ganglion cells and astrocytes were co-cultured for four days, and then treated with NGF and each compound (Comp. A, Comp. B). 27 hours later, nuclei were stained with DRAQ5 (red) and neurons with Alexa 488-recognizing anti-Tuj1 antibody (green). Neurite area was identified and quantified from Tuj1-stained images. Operetta CLS and Opera Phenix Plus enable high-speed, high-resolution image analysis of samples treated with compounds at various concentrations, allowing the neurotoxicity of compounds to be determined using the reduction in neurite area as an indicator.
Others
Tests can also be performed using siRNA-based gene knockdown studies and forced gene expression tests.
For suspension cells, analysis using a flow cytometer is more effective.
References
●Quantification of oligodendrocyte differentiation and MBP levels in tissue sections
Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo
Najm FJ et al., Nature 2015
●Quantitative Quantification of Myotube Differentiation
Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentitation
Polesskaya A et al., PlosOne 2013
●Confirmation of spheroid formation
Evaluation of chemotherapeutics in a three-dimensional breast cancer model
Lovitt CJ et al., J Cancer Res Clin Oncol. 2015
●Screening Methods Using Protein-Protein Interactions
Visualization and targeted disruption of protein interactions in living cells
Herce HD et al., Nat Communications 2013
●Measuring Protein-Protein Interactions with BRET
Nanoluciferase Signal Brightness Using Furimazine Substrates Opens Bioluminescence Resonance Energy Transfer to Widefield Microscopy
Jiho Kim, Regis Grailhe., Cytometry A. 2016
*Studies will be mainly conducted by those with doctoral degrees.
