Study Overview
Simply put, a High-content Screening System is a device capable of quantifying fluorescent images of cells.
It is also called High Content Analysis (HCA) or High Content Imaging (HCI) .
Please feel free to contact us.
Equipment used
Operetta CLS (Revvity)
Opera Phenix Plus (Revvity)
Image analysis application examples
Just like the example above, there are various applications.
Accurate cell recognition
The accurate recognition of the nucleus, cytoplasm, and intracellular spots is the foundation of image analysis in High Content Analysis.
Myotubular differentiation
This is an experiment of myotubular differentiation.
No sample was added to the medium as a control.
Myogenic differentiation factor was added as a positive control.
It is clear in the image.
Myoblast to myotube cell differentiation is promoted by myogenic differentiation factor.
Neurite outgrowth
This is an experiment of neurite outgrowth.
Neurotrophic factors promote neurite outgrowth.
The fluorescent image also clearly shows neurite outgrowth, but also numerically.
The number of neurites is the total length of neurites per cell.
Others
Tests can also be performed using siRNA-based gene knockdown studies and forced gene expression tests.
For suspension cells, analysis using a flow cytometer is more effective.
References
●Growth Rate inhibition metrics correct for confounders in measuring sensitivity to cancer drug
Hafner et al., Nature Method, 2016
●A high-conten image-based metthof for quantitatively studying context-dependenta cell population dynamics
Garvey et al., Scientific Reports, 2016
●Anticancer drug released from near IR-activated prodrug overcomes spatiotemporal limits of singlet oxgen
Rajaputra et al., Bioorganic & Med. Chem., 2016
●A Comprehensive Analysis of the Dynamic Response to Aphidicolin-Mediated Replication Stress Uncovers Targets fot ATM and ATMIN
Mazouzi et al., Cell Rep., 2016
●PPIP5K1 Suppresses Etoposide-triggered Apoptosis
Machkalyan et al., J. Molecular Signaling, 2016
*Studies will be mainly conducted by those with doctoral degrees.
