Study Overview
The anti-inflammatory effects of test substances are evaluated using various cell types.
As an example, we will introduce a test using J774.1 cells (a mouse-derived macrophage cell line). Inflammatory cells such as macrophages produce inflammatory cytokines (such as TNF-α and IL-6) in response to LPS. TNF-α and IL-6 promote the differentiation of osteoclast precursor cells into osteoclasts, but excessive production of these substances induces bone resorption (bone breakdown) by osteoclasts, leading to osteoporosis and rheumatoid arthritis. Furthermore, it has been reported that TNF-α accelerates cellular dysfunction in hepatocytes, leading to liver cirrhosis and liver failure. Therefore, we conduct an inflammation suppression study to search for new substances that inhibit TNF-α production.
Study example
Basic Experimental design
| Cells used | Human PBMC (peripheral blood mononuclear cells) Macrophage cell lines (e.g., J774.1) Normal human epidermal cells, etc. |
|---|---|
| Measurement items | Cytokine production measurement (ELISA, multiplex measurement) |
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of cytokine production after LPS stimulation |
*Studies will be mainly conducted by those with doctoral degrees.
