Study Overview
Exercise-induced fat burning occurs primarily in skeletal muscle cells and brown fat cells. These cells are rich in mitochondria, which metabolize sugars and fats, converting them into energy and heat. Therefore, activating mitochondrial function in skeletal muscle cells and brown fat cells promotes fat burning.
In this study, we will measure mitochondrial activity and mitochondrial quantity within cells to verify the mitochondrial activation effects of the test substance.
If you would like to evaluate mitochondrial metabolic function more closely, please consider metabolic analysis using an extracellular flux analyzer.
Study example (Mito Tracker/Hoechst staining of myoblasts and myotubes)
Basic Experimental design
| Cells used | Myotube cells, adipocytes, etc. Various other cells |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Mitochondrial activity measurement (MTT method, etc.) Mitochondrial quantification (Mito Tracker staining) Live cell count measurement (Hoechst staining) ATP production measurement (optional) |
*Studies will be mainly conducted by those with doctoral degrees.
