Study Overview
Fat burning during exercise mainly occurs in skeletal muscle and brown fat cells because they are rich in mitochondria. Since mitochondria convert glucose and fatty acids into energy and heat, activating their function in these cells promotes fat loss. Substances that promote mitochondrial activation can lead to energy consumption, thereby burning fat in the body.
In this assay, we evaluate test substances for effects related to mitochondrial activation and quantity within cells.
For assessments related to mitochondrial metabolic function, we recommend metabolic analysis using extracellular flux analyzer.
Study example (Mito Tracker/Hoechst staining of myoblasts and myotubes)
Basic Experimental design
| Cells used | Myotube cells, adipocytes, etc. Various other cells |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Mitochondrial activity measurement (MTT method, etc.) Mitochondrial quantification (Mito Tracker staining) Live cell count measurement (Hoechst staining) ATP production measurement (optional) |
*All our studies are mainly performed and handled by our team of PhD scientists.
