Study Overview
When harmful substances such as bacteria or viruses enter the body, immune cells like macrophages detect them and release different signaling molecules called cytokines to start the body’s defense response. These cytokines activate B cells and T cells, which help the body fight infection and produce antibodies. At the same time, inflammatory cytokines help remove infected cells and speed up metabolism around the infected area. However, if this response becomes too strong, it can cause excessive inflammation and damage to the body.
This study will examine the effect of the test substance on cytokine production in macrophages, which play a central role in the immune response.
Basic Experimental design
| Cells used | Human monocytic cell line (THP-1) Human PBMC, etc. |
|---|---|
| Measurement items | Cytokine production measurement (ELISA, multiplex analysis) |
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of cytokine production after LPS stimulation |
*Studies will be mainly conducted by those with doctoral degrees.
