Study Overview
Using suspension bead array technology, we can measure many kinds of proteins related to the body’s reactions, such as immune and inflammation signals, at the same time from a very small sample. Up to 100 different targets can be checked in one test. We can use many types of samples, including blood, spinal fluid, cell culture liquid, and cell extracts.
This method is especially useful for studying substances released from cells, such as signals related to immunity, inflammation, fat tissue, and muscle activity. We can also support the entire study process, from growing immune cells such as human blood-derived cells and macrophage cells, to adding test substances and measuring the released signals.
Equipment used
Bio-Plex 200 (Bio-Rad)
Features
With a normal ELISA test, only one protein can be measured at a time.
This means that if you want to measure 100 different proteins, you need to perform 100 separate tests and use 100 different ELISA kits.
However, multiplex analysis can measure up to 100 different proteins at the same time in a single test using only a small amount of sample.
Principle
Measurement kits and items
Compatible Kit
- Bio-Rad
- Merck
- R&D systems
- Thermo Fisher Scientific
Measurable items
- Cytokines, chemokines, growth factors
- Inflammatory factors
- Factors involved in intracellular signaling (phosphorylation, apoptosis, etc.)
- Diabetes-related factors
- Isotyping
- Factors related to acute responses
- Nephrotoxicity/injury, etc.
References
The angiogenetic process as a theraputic target in cancer
Bridges EM et al., Biochem Pharmacol. 2011
Second generation multiplex immunoassays
Dale E et al., Bioradiations 2008
Regulatory T cells confer a circadian signature on inflammatory arthritis
L. E. Hand et. al., Nature Communications 2020
*Studies will be mainly conducted by those with doctoral degrees.
