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Real-time PCR (gene expression analysis)

Study Overview

Cells often respond to test substances by up- or down regulating genes, subsequently influencing the physiological state of the cell. In order to understand which and how much the gene expression changes, RNA needs to be collected from the cells after a test substance is administered. Using real-time PCR (RT-PCR), the expression levels of the gene of interest can be measured. Multiple samples can be evaluated simultaneously for high-throughput screening and large-scale testing.

We can also perform gene expression analysis on RNA and cDNA samples that you already have available. If you only have RNA samples you would like to send us for testing, we will first generate cDNA from the RNA for RT-PCR. We can also design the primers, or use primers that you provide for RT-PCR.

Equipment information

Study Example

In this example, we measured the expression level of the NANOG gene, which is a marker of undifferentiated iPS cells
GAPDH was used as an internal control gene.

Basic Experimental design

Cells used	Various cells
Measurable items	Gene expression analysis using real-time PCR Intercalation method (SYBR Green method) or probe method (TaqMan probe method) MicroRNA measurement PCR array, etc.
Preliminary study	Cytotoxicity assay
Main study	Various gene expression analyses

*All our studies are mainly performed and handled by our team of PhD scientists.