CRO｜Real-time PCR (gene expression analysis)

Study Overview

Your target cells are cultured with the test substance. After that, RNA is collected from the cells, and the activity of the target gene is measured using real-time PCR. We can also handle many samples at the same time for screening and large-scale testing.

You can also send us your own RNA or cDNA samples and request only the gene expression analysis. If you send RNA samples, we will first make cDNA and then perform the real-time PCR test. We can also design primers for you, or we can use primers that you provide.

使用機器

Study Example

We measured the expression level of the NANOG gene, a marker of undifferentiated iPS cells.
GAPDH was used as an internal control gene.

Basic Experimental design

Cells used	Various cells
Measurable items	Gene expression analysis using real-time PCR Intercalation method (SYBR Green method) or probe method (TaqMan probe method) MicroRNA measurement PCR array, etc.
Preliminary study	Cytotoxicity assay
Main study	Various gene expression analyses

*Studies will be mainly conducted by those with doctoral degrees.