Study Overview
We use the CytoFLEX SRT cell sorter made by Beckman Coulter. A cell sorter is a machine that can find and separate specific types of cells from a mixture of many cells. The separated cells can then be studied further or grown again in culture. For example, we are able to separate rare cells found in blood and tissue samples, which can be used for different testing applications.
As part of the cell sorting workflow, the cell sorter can be used together with CytoFLEX S for identification prior to sorting.
Equipment used
CytoFLEX SRT (Beckman Coulter)
Features of the CytoFLEX SRT
① Highly sensitive detection for tests involving multiple colors
② Able to separate cells into four groups using different sorting methods
③ High cell recovery and viability after sorting
④ Accurate cell collection and information about each cell is preserved
Analysis examples (T cell sorting using human whole blood)
Typical study examples
●Gene Introduction and Cell Sorting
During the gene transfection process, not all cells take up genes. In order to identify and isolate the cells which have been successfully transfected, a flow cytometer can be first used to identify the cells, followed by isolation and collection with the cell sorter.
●Single Cell Sorting
Single cell sorting is also possible for collecting information about the features of individual cells.
●Blood, Tissue, and Cancer Tissue
Blood, Tissue and Cancer samples generally contain a mixture of different cells and cell types. Using a cell sorter, we can isolate certain cell populations of interest. These cells can be further studied or used for additional testing.
*All our studies are mainly performed and handled by our team of PhD scientists.
