Study Overview
Flow cytometry is a method that uses laser light to examine cells. It can quickly measure and analyze many cells one by one by detecting light and fluorescent signals from the cells. This makes it possible to study different features of cells in a large group of cells at the same time.
Many types of cell analysis can be performed, including the examples shown below.
In addition to providing flow cytometry testing using samples supplied by customers, we also offer custom cell-based assay services. We can support the entire study process, from culturing different kinds of cells, to adding test substances, and finally analyzing the cells with flow cytometry.
We also provide services using a cell sorter, a special type of flow cytometer that can separate specific cells from a mixture of cells.
Please feel free to contact us.
Equipment used
CytoFLEX S (Beckman Coulter)
Features of the CytoFLEX S
- Measure a 96-well plate in 32 minutes
- Switch between single tube and plate formats in just 5 minutes
- Supports analysis using a plate loader
- Bead assay enables simultaneous measurement of various cytokines
Typical analysis examples
- Cell cycle
- Apoptosis
- Cell surface antigens (e.g., CD markers)
- Immune cell assessment (lymphocyte subset analysis)
- Intracellular cytokine analysis
- Multiplex quantification of multiple cytokines in culture supernatant and serum
- Detection and quantification of various biomarkers
- Analysis of stem cell differentiation status
CytoFLEX S Specifications
| Laser | Fluorescence Channel |
|---|---|
| Violet 405 nm | 450/45 BP |
| 525/40 BP | |
| 610/20 BP | |
| 660/20 BP | |
| Blue 488 nm | 525/40 BP |
| 690/50 BP | |
| Yellow 561 nm | 585/42 BP |
| 610/20 BP | |
| 690/50 BP | |
| 780/60 BP | |
| Red 638 nm | 660/20 BP |
| 712/25 BP | |
| 780/60 BP |
*The wavelength can be changed by inserting a filter.
Main fluorescent dyes that can be analyzed
Pacific Blue dye, V450, eFluor450, BV421, Krome Orange, AmCyan, V500, BV510, BV605, Qdot 605, BV650, Qdot655, FITC, Alexa Fluor488, CFSE, Fluo-3, PC5.5, PC5, PerCP, PerCP-Cy5.5, PI, PE, DsRED, tdTomato, ECD, PE-Texas Red, PE-CF594, mCherry, PC7, APC, Alexa Fluor647, eFluor660, APC-A700, Alexa Fluor700, APC-A750, APC Cy7, APC-H7, APC eFluor750 など
References
Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
Wagner et al., Nature Communications 2020
A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles
Brittain IV et al., Scientific Reports 2019
*Studies will be mainly conducted by those with doctoral degrees.
