Study Overview
Flow cytometry is a laser-based laboratory technique used to analyze the physical and biochemical characteristics from fluorescent signals of cells or particles as they flow in a fluid stream. It measures cell size, internal complexity, and targeted protein expression, allowing researchers to quickly evaluate several of such parameters across thousands of individual cells.Many types of cell analysis can be performed, including the examples shown below.
While standalone flow cytometry analysis can be performed at ACEL with your samples, we can also perform this as part of an entire study. This can start from cell culture, addition of test substances, and finally followed by flow cytometry.
We are also able to perform cell sorting using a special type of flow cytometer. This allows you to isolate specific cells from a mixture for downstream testing or characterization.
If you would like more information on this, please feel free to contact us.
Equipment used
CytoFLEX S (Beckman Coulter)
Features of the CytoFLEX S
- Measure a 96-well plate in 32 minutes
- Switch between single tube and plate formats in just 5 minutes
- Supports semi-automated analysis using a plate loader, increasing speed.
- Bead assay enables simultaneous measurement of various cytokines
Typical analysis examples
- Cell cycle
- Apoptosis
- Cell surface antigens (e.g., CD markers)
- Immune cell assessment (lymphocyte subset analysis)
- Intracellular cytokine analysis
- Multiplex quantification of multiple cytokines in culture supernatant and serum
- Detection and quantification of various biomarkers
- Analysis of stem cell differentiation status
CytoFLEX S Specifications
| Laser | Fluorescence Channel |
|---|---|
| Violet 405 nm | 450/45 BP |
| 525/40 BP | |
| 610/20 BP | |
| 660/20 BP | |
| Blue 488 nm | 525/40 BP |
| 690/50 BP | |
| Yellow 561 nm | 585/42 BP |
| 610/20 BP | |
| 690/50 BP | |
| 780/60 BP | |
| Red 638 nm | 660/20 BP |
| 712/25 BP | |
| 780/60 BP |
*The wavelength can be changed by inserting a filter.
Main fluorescent dyes that can be analyzed
Pacific Blue dye, V450, eFluor450, BV421, Krome Orange, AmCyan, V500, BV510, BV605, Qdot 605, BV650, Qdot655, FITC, Alexa Fluor488, CFSE, Fluo-3, PC5.5, PC5, PerCP, PerCP-Cy5.5, PI, PE, DsRED, tdTomato, ECD, PE-Texas Red, PE-CF594, mCherry, PC7, APC, Alexa Fluor647, eFluor660, APC-A700, Alexa Fluor700, APC-A750, APC Cy7, APC-H7, APC eFluor750 etc
References
Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
Wagner et al., Nature Communications 2020
A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles
Brittain IV et al., Scientific Reports 2019
*All our studies are mainly performed and handled by our team of PhD scientists.
