Study Overview
In this study, we use a fluorescence microscope to capture images of cells and other samples, obtaining visually compelling data for use in promotional materials. We treat cells with test substances and perform a variety of evaluations, including intracellular localization of target molecules, qualitative and quantitative analysis, cell differentiation, cell activation, and cytotoxicity. We also offer time-lapse imaging. Our experienced researchers will flexibly propose test systems to suit your test objectives, so please feel free to contact us.
In addition, we own a variety of equipment, including fluorescent plate readers and high-content screening instruments, and we also offer custom-made testing services that combine these instruments.
Microscope used
List of Studies
| Study items | Staining Target・ Staining Method | Study purpose |
|---|---|---|
| Various immunostaining | Various targets | Verification of protein expression levels, cell differentiation observation, intracellular localization, etc. |
| DNA staining | DAPI | Verification of cell damage by nuclear staining of dead cells |
| Hoechst | Verification of cell damage by nuclear staining of live cells | |
| RNA staining | Newly synthesized RNA RNA-specific dyes etc. | Verify the effect of test substances on transcriptional activity |
| Organelle/ cell structure staining | Mitochondria | Verification of intracellular localization, and verification of mitochondrial activity and quantity |
| Endoplasmic reticulum | Verification of intracellular localization, etc. | |
| Golgi apparatus | Verification of intracellular localization and observation of morphogenetic changes | |
| Lysosomes | Verification of intracellular localization, etc. | |
| Cell membrane | Verification of intracellular localization and neurite outgrowth activity | |
| Adiposomes | Fat storage research using neutral fat staining, and verification of toxicity and side effects of test substances | |
| Intercellular junctions | Verification of cell-cell binding activity, including cell function activation and degradation | |
| Cytoskeleton | Actin, tubulin, etc. | Verification of intracellular localization and cytotoxicity of test substances |
| Living cells/ dead cells | Calcein AM | Verify the cytotoxicity and cell activation of test substances |
| Cell Tracker | ||
| BrdU staining | Verify cell division by staining cells during DNA synthesis | |
| Caspase Reagents | Verify the presence of apoptosis and necrosis | |
| TUNEL assay, etc. | ||
| Oxidative stress | Mitochondrial activity | Verification of oxidative stress by the presence or absence of reactive oxygen species generated from mitochondria |
| Nitric oxide | Verification of oxidative stress and signaling depending on the presence or absence of nitric oxide | |
| Reactive oxygen | Verification of antioxidant capacity based on the presence or absence of reactive oxygen species | |
| Thiol Reaction Reagents | Observation of intracellular redox state and verification of oxidative stress status | |
| Autophagy | Anti-LC3B staining, Lysosomal staining, etc. | Verification of the autophagosome formation promoting effect |
| Various ion concentration | Various ion indicators such as Ca2+ and H+ | Measurement of intracellular ion concentrations |
| Subcellular localization | GFP, YFP, RFP, etc. | Intracellular localization, intracellular movement/transport of various fluorescent fusion proteins |
*Studies will be mainly conducted by those with doctoral degrees.
