Fluorescence observation of cells using a fluorescence microscope

Study objectives

Bioimaging technology has advanced.
Fluorescent observation of cells has made it possible to visually understand various biological phenomena.
Studies are conducted by adding test substances to various cells and analyzing the images.
A wide range of studies are available, from intracellular localization and qualitative and quantitative measurements of target factors to evaluations of cell differentiation, cell activation, and cytotoxicity.

We also carry fluorescent plate readers. Combining our equipment with a fluorescent plate reader may enable further analysis.

We also offer time-lapse imaging (changes over time).

We offer high-content screening for screening tests involving multiple samples or when more advanced analysis is required.

We also offer services for visually compelling data to supplement or reinforce existing data, or for use as promotional materials.

List of Bioimaging tests

Fluorescence microscope model

Keyence All-in-One Fluorescence Microscope (BZ-X700)

Study items	Staining Target・Staining Method	Study purpose
Various immunostaining	Various targets	Verification of protein expression levels, cell differentiation observation, intracellular localization, etc.
DNA staining	DAPI	Verification of cell damage by nuclear staining of dead cells
	Hoechst	Verification of cell damage by nuclear staining of live cells
RNA staining	Newly synthesized RNA RNA-specific dyes etc.	Verify the effect of test substances on transcriptional activity
Organelle/cell structure staining	Mitochondria	Verification of intracellular localization, and verification of mitochondrial activity and quantity
	Endoplasmic reticulum	Verification of intracellular localization, etc.
	Golgi apparatus	Verification of intracellular localization and observation of morphogenetic changes
	Lysosomes	Verification of intracellular localization, etc.
	Cell membrane	Verification of intracellular localization and neurite outgrowth activity
	Adiposomes	Fat storage research using neutral fat staining, and verification of toxicity and side effects of test substances
	Intercellular junctions	Verification of cell-cell binding activity, including cell function activation and degradation
Cytoskeleton	Actin, tubulin, etc.	Verification of intracellular localization and cytotoxicity of test substances
Living cells/dead cells	Calcein AM	Verify the cytotoxicity and cell activation of test substances
	Cell Tracker
	BrdU staining	Verify cell division by staining cells during DNA synthesis
	Caspase Reagents	Verify the presence of apoptosis and necrosis
	TUNEL assay, etc.
Oxidative stress	Mitochondrial activity	Verification of oxidative stress by the presence or absence of reactive oxygen species generated from mitochondria
	Nitric oxide	Verification of oxidative stress and signaling depending on the presence or absence of nitric oxide
	Reactive oxygen	Verification of antioxidant capacity based on the presence or absence of reactive oxygen species
	Thiol Reaction Reagents	Observation of intracellular redox state and verification of oxidative stress status
Autophagy	Anti-LC3B staining, Lysosomal staining, etc.	Verification of the autophagosome formation promoting effect
Various ion concentrations	Various ion indicators such as Ca2+ and H+	Measurement of intracellular ion concentrations
Subcellular localization	GFP, YFP, RFP, etc.	Intracellular localization, intracellular movement/transport of various fluorescent fusion proteins

*Studies will be mainly conducted by those with doctoral degrees.