Study Overview
Using Olink’s PEA makes it possible to comprehensively quantify proteins.
We use Olink to comprehensively quantify proteins in samples provided by customers.
・This is a contract service in which we take your biological samples and perform comprehensive protein quantitative analysis (proteome analysis) using Olink’s technology (PEA).
・A variety of sample formats can be measured, including serum, plasma (EDTA/citrate/heparin), tissue lysate, cell culture supernatant, urine, cerebrospinal fluid, synovial fluid, small biological samples (fine needle biopsy, dried blood spot, etc.), cell lysate, and extracellular vesicles.
Characteristics of quantification by PEA
“PEA (Proximity Extension Assay)” is a method that uses an oligonucleotide-labeled antibody pair against a target protein.
The two antibodies bind to the same target in close proximity, allowing the oligos to hybridize.
From there, the DNA is extended and read using real-time PCR or NGS (next-generation sequencing).
This allows for the simultaneous, highly sensitive measurement of multiple proteins using very small sample volumes (approximately 1–6 µL).
Its major strength is its high specificity and quantification.
Plan
There are three plans available:
| Plan | Key Features |
| Olink Explore HT | Targets over 5,400 proteins. Boasts a wide dynamic range and high specificity (99.5%). Sample volume is 2 µL. |
| Olink Target 96 / Target 48 | 43-92 protein panel. 1 µL sample volume. Supports both absolute and relative quantification. Rigorously validated. |
| Olink Flex & Focus | Small panel of 15–21 targets. Sample volume 1 µL. Supports both absolute and relative quantitation. Rigorously validated. |
Application to cell-based assay
Your sample is added to various cultured cells, and the sample is recovered from the culture supernatant or the cells themselves.
It is also possible to quantify proteins in the culture supernatant or extracts from the cells themselves using Olink’s proteome analysis.
Please feel free to contact us.
*Studies will be mainly conducted by those with doctoral degrees.
