Study Overview
Using Olink technology, we can measure many proteins in a sample at the same time.
We provide a service in which we receive customer samples and analyze many kinds of proteins using Olink’s PEA technology.
・We can test many types of samples, including blood serum, plasma, tissue samples, cell culture liquid, urine, cerebrospinal fluid, joint fluid, very small biological samples (such as fine needle biopsy samples and dried blood spots), cell samples, and extracellular vesicles.
Characteristics of quantification by PEA
“PEA (Proximity Extension Assay)” is a method that uses an oligonucleotide-labeled antibody pair against a target protein.
The two antibodies bind to the same target in close proximity, allowing the oligos to hybridize.
From there, the DNA is extended and read using real-time PCR or NGS (next-generation sequencing).
This allows for the simultaneous, highly sensitive measurement of multiple proteins using very small sample volumes (approximately 1–6 µL).
Its major strength is its high specificity and quantification.
Plan
There are three plans available:
| Plan | Key Features |
| Olink Explore HT | Targets over 5,400 proteins. Boasts a wide dynamic range and high specificity (99.5%). Sample volume is 2 µL. |
| Olink Reveal | 1,034 different proteins. Our focus is on immunity and inflammation. For serum and plasma samples, a minimum sample volume of 20 μL is required. Analysis is performed using a next-generation sequencer (NovaSeq 600 system). |
| Olink Target 96 / Target 48 | 43-92 protein panel. 1 µL sample volume. Supports both absolute and relative quantification. Rigorously validated. |
| Olink Flex & Focus | Small panel of 15–21 targets. Sample volume 1 µL. Supports both absolute and relative quantitation. Rigorously validated. |
Application to cell-based assay
Your sample is added to various cultured cells, and the sample is recovered from the culture supernatant or the cells themselves.
It is also possible to quantify proteins in the culture supernatant or extracts from the cells themselves using Olink’s proteome analysis.
Please feel free to contact us.
*Studies will be mainly conducted by those with doctoral degrees.
