Study Overview
We perform proteome analysis using olink’s PEA technology.
We offer two methods: one using a next generation sequencer (NovaSeq 600 system) and another using a real-time PCR instrument (Signature Q100).
While the next generation sequencer allows for the analysis of large volumes of samples at once, it is expensive.
Real-time PCR offers a more cost-effective analysis option.
For real-time PCR analysis, we also offer plans that allow for absolute quantification.
We can analyze a wide range of biological samples, including serum and plasma.
Characteristics of quantification by PEA
“PEA (Proximity Extension Assay)” is a method that uses an oligonucleotide-labeled antibody pair against a target protein.
The two antibodies bind to the same target in close proximity, allowing the oligos to hybridize.
From there, the DNA is extended and read using real-time PCR or NGS (next generation sequencing).
This allows for the simultaneous, highly sensitive measurement of multiple proteins using very small sample volumes (approximately 1–6 µL).
Its major strength is its high specificity and quantification.
Plan
There are three plans available:
| Plan | Key Features |
| Olink Explore HT | It targets over 5,400 types of proteins. It boasts a wide dynamic range and high specificity (99.5%). The sample volume is 2 µL. Analysis is performed using a next-generation sequencer (NovaSeq 600 system). |
| Olink Reveal | Protein panels containing 43 to 92 proteins. Sample volume: 1 µL. Supports both absolute and relative quantification. Rigorously validated. Analysis is performed using a dedicated real-time PCR instrument (Signature Q100). |
| Olink Target 96 / Target 48 | Can measure 43 to 92 proteins in a single test. Only 1 µL of sample is required. It can measure the actual amount of proteins and also compare differences between samples. The test method has been carefully checked to provide reliable results. |
| Olink Flex & Focus | A small-scale panel consisting of 15 to 21 targets. Sample volume is 1 µL. Supports both absolute and relative quantification. Rigorously validated. Analysis is performed using a dedicated real-time PCR instrument (Signature Q100). |
Application to cell-based assay
Olink analysis is also available for cell-based assays.
Proteome analysis is possible by analyzing cell culture supernatants using Olink’s PEA technology.
Please feel free to contact us.
*Studies will be mainly conducted by those with doctoral degrees.
