Study Overview
Using Olink technology, we can measure many proteins in a sample at the same time.
We provide a service in which we receive customer samples and analyze many kinds of proteins using Olink’s PEA technology.
・We can test many types of samples, including blood serum, plasma, tissue samples, cell culture liquid, urine, cerebrospinal fluid, joint fluid, very small biological samples (such as fine needle biopsy samples and dried blood spots), cell samples, and extracellular vesicles.
Characteristics of quantification by PEA
“PEA (Proximity Extension Assay)” is a method that uses an oligonucleotide-labeled antibody pair against a target protein.
The two antibodies bind to the same target in close proximity, allowing the oligos to hybridize.
From there, the DNA is extended and read using real-time PCR or NGS (next-generation sequencing).
This allows for the simultaneous, highly sensitive measurement of multiple proteins using very small sample volumes (approximately 1–6 µL).
Its major strength is its high specificity and quantification.
Plan
There are three plans available:
| Plan | Key Features |
| Olink Explore HT | Can measure more than 5,400 proteins at the same time. It can detect both very small and very large changes with high accuracy. Only 2 µL of sample is needed. |
| Olink Reveal | Can measure 1,034 proteins related mainly to the immune system and inflammation. For blood serum or plasma samples, at least 20 µL of sample is needed. Analysis is performed using a high-performance DNA analysis system (NovaSeq 600). |
| Olink Target 96 / Target 48 | Can measure 43 to 92 proteins in one test. Only 1 µL of sample is needed. It supports both exact amount measurement and comparison between samples. The test method has been carefully verified for reliability. |
| Olink Flex & Focus | Can measure a small number of selected proteins (15–21 targets). Only 1 µL of sample is needed. It supports both exact amount measurement and comparison between samples. The test method has been carefully verified for reliability. |
Application to cell-based assay
Your sample is added to various cultured cells, and the sample is recovered from the culture supernatant or the cells themselves.
It is also possible to quantify proteins in the culture supernatant or extracts from the cells themselves using Olink’s proteome analysis.
Please feel free to contact us.
*Studies will be mainly conducted by those with doctoral degrees.
