Study Overview
Flow cytometry is an analytical method that uses a laser to detect fluorescent signals and other signals. It allows for simultaneous, high-speed analysis and quantification of various characteristics of individual cells in a large cell population. A variety of analyses are possible, including the examples below.
In addition to offering contract flow cytometry analysis services using samples provided by our clients, we also provide custom cell-based assay services. We can conduct integrated testing from various cell cultures, through test substance treatment, to flow cytometry analysis.
We also offer analysis services using a cell sorter, a type of flow cytometer that separates cells.
Please feel free to contact us.
Equipment used
CytoFLEX S (Beckman Coulter)
Features of the CytoFLEX S
- Measure a 96-well plate in 32 minutes
- Switch between single tube and plate formats in just 5 minutes
- Supports analysis using a plate loader
- Bead assay enables simultaneous measurement of various cytokines
Typical analysis examples
- Cell cycle
- Apoptosis
- Cell surface antigens (e.g., CD markers)
- Immune cell assessment (lymphocyte subset analysis)
- Intracellular cytokine analysis
- Multiplex quantification of multiple cytokines in culture supernatant and serum
- Detection and quantification of various biomarkers
- Analysis of stem cell differentiation status
CytoFLEX S Specifications
| Laser | Fluorescence Channel |
|---|---|
| Violet 405 nm | 450/45 BP |
| 525/40 BP | |
| 610/20 BP | |
| 660/20 BP | |
| Blue 488 nm | 525/40 BP |
| 690/50 BP | |
| Yellow 561 nm | 585/42 BP |
| 610/20 BP | |
| 690/50 BP | |
| 780/60 BP | |
| Red 638 nm | 660/20 BP |
| 712/25 BP | |
| 780/60 BP |
*The wavelength can be changed by inserting a filter.
Main fluorescent dyes that can be analyzed
Pacific Blue dye, V450, eFluor450, BV421, Krome Orange, AmCyan, V500, BV510, BV605, Qdot 605, BV650, Qdot655, FITC, Alexa Fluor488, CFSE, Fluo-3, PC5.5, PC5, PerCP, PerCP-Cy5.5, PI, PE, DsRED, tdTomato, ECD, PE-Texas Red, PE-CF594, mCherry, PC7, APC, Alexa Fluor647, eFluor660, APC-A700, Alexa Fluor700, APC-A750, APC Cy7, APC-H7, APC eFluor750 など
References
Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
Wagner et al., Nature Communications 2020
A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles
Brittain IV et al., Scientific Reports 2019
*Studies will be mainly conducted by those with doctoral degrees.
