Real-time PCR (gene expression analysis)

Study Overview

Your desired cell type is cultured in the presence of the test substance. RNA is extracted from the cells, and the expression level of the target gene is measured using real-time PCR. We can also accommodate requests for multiple samples, such as screening, and high-throughput analysis.

You can also send us your own prepared RNA or cDNA and request gene expression analysis only. If you send us your RNA, we will convert it to cDNA and perform real-time PCR analysis. We can also design primers for you. We are also happy to perform analysis using primers of your choice.

使用機器

Study Example

We measured the expression level of the NANOG gene, a marker of undifferentiated iPS cells.
GAPDH was used as an internal control gene.

Basic Experimental design

Cells used	Various cells
Measurable items	Gene expression analysis using real-time PCR Intercalation method (SYBR Green method) or probe method (TaqMan probe method) MicroRNA measurement PCR array, etc.
Preliminary study	Cytotoxicity assay
Main study	Various gene expression analyses

*Studies will be mainly conducted by those with doctoral degrees.