Study Overview
Skin and eyes are always exposed to dry conditions. The cells on the surface of the skin and eyes help protect the inside of the tissue from drying out by connecting tightly to each other and forming a strong barrier. Because of this, the condition of these cell connections is an important sign of how well the tissue can protect itself from dryness.
In this study, we will look at a protein called occludin, which helps cells stay tightly connected. By checking the condition of these connections after dry stress is applied, we can evaluate whether the test substance may help improve dry skin and dry eye conditions.
Occludin antibody staining in immortalized human corneal epithelial cells
Basic Experimental design
| Cells used | Immortalized human corneal epithelial cells, human epidermal keratinocytes, etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Live cell count (WST-8 method) Occludin antibody staining |
*Studies will be mainly conducted by those with doctoral degrees.
