Study Overview
Melanin, produced by melanocyte cells, is also known as melanin pigment and is known to be the cause of skin darkening (pigmentation). Reducing melanin production is expected to have a whitening effect, making it a focus of cosmetic development.
This study will verify the melanin production-inhibiting effects of test substances using mouse-derived B16 melanoma cells and human melanocytes, with the aim of verifying melanin inhibitors that are expected to have a skin whitening effect and exploring new functional substances.
Cell image of melanin production in B16 cells (negative control)
Cell image under PTU-added conditions (positive control)
Study example
Basic Experimental design
| Cells used | Mouse-derived B16 melanoma cells, human melanocytes |
|---|---|
| Culture conditions | Theophylline added |
| Measurement items | Intracellular melanin content |
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of intracellular melanin content |
*Studies will be mainly conducted by those with doctoral degrees.
