Study Overview
Melanin, produced by melanocytes, is the pigment responsible for skin darkening. Because inhibiting its production can lead to a whitening effect, it is a primary focus in cosmetic development.
This study evaluates the ability of a test substance to inhibit the production of melanin. This can be performed in both mouse B16 melanoma cells or human melanocytes to identify potential melanin inhibitors as skin whitening and novel functional substances. We also offer a 3D skin model for assessing melanin production. Please click here for more information.
Cell image of melanin production in B16 cells (negative control)
Cell image under cells incubated with PTU (positive control)
Study example
Basic Experimental design
| Cells used | Mouse-derived B16 melanoma cells, human melanocytes |
|---|---|
| Culture conditions | Theophylline added |
| Measurement items | Intracellular melanin content |
| Preliminary study | Cytotoxicity assay |
| Main study | Measurement of intracellular melanin content using alkaline lysis |
*All our studies are mainly performed and handled by our team of PhD scientists.
