Study Overview
Genes are typically regulated so that they are expressed in the required amounts when needed. However, forcing specific genes into cells to overexpress can change the cell’s properties. Using these cells can help elucidate the function of genes. Furthermore, by introducing immortalization or disease-related genes into cells, immortalized cells and disease model cells can be established. These cells can be used in a variety of fields, including drug discovery screening.
We perform forced gene expression tests for a variety of genes according to customer needs. We can handle both transient and stable expression cells, and will propose an assay system that best suits the purpose of the study. We also offer various evaluation tests using the gene-overexpressing cells we have created. (*)
*This test involves forced gene expression and confirmation of that expression. Confirmation of transformed cells (immortalization, disease reproducibility, etc.) is performed separately.
Examples of applications to cell testing:
- Gene function analysis
- Gene expression suppression by overexpression of shRNA-expressing DNA
- Cell transformation (recombinant protein-producing cells, immortalized cells, disease model cell establishment, etc.)
- Reporter assays, etc.
Transient and stable expression
There are two methods for introducing and expressing genes into cells, as shown in the diagram above.
One is transient gene expression. In this method, the introduced gene is not generally integrated into genomic DNA (chromosomal DNA). As a result, the introduced gene gradually decreases with repeated cell division. This method is suitable for evaluating the effects of temporarily introduced genes, such as in reporter assays and transient knockdown.
The other is stable gene expression. In this method, the introduced gene is integrated into genomic DNA. As a result, gene expression is stable even with repeated cell division. This method is suitable for continuously evaluating forced-expression cells, such as establishing forced-expression cell lines.
We can accommodate both transient and stable expression assay systems to suit your study objectives.
*Studies will be mainly conducted by those with doctoral degrees.
