Study Overview
Genes in cells are usually controlled so they work at the right time and in the right amount. However, by adding a specific gene into cells and making it work more strongly, the behavior and characteristics of the cells can change. Studying these cells helps us better understand what a gene does. In addition, by adding genes related to cell growth or disease, it is possible to create long-lasting cells and disease model cells. These cells can be used in many research areas, including drug discovery screening.
We provide gene overexpression studies for many types of genes based on customer needs. We can prepare both temporary expression cells and long-term stable expression cells, and we will suggest the most suitable test system for your research purpose. We also offer different evaluation tests using the cells we create with enhanced gene expression. (*)
*This service includes introducing genes into cells and checking whether the genes are expressed correctly. Additional confirmation of cell transformation, such as immortalization or disease-like characteristics, is provided separately.
Examples of applications to cell testing:
- Gene function analysis
- Gene expression suppression by overexpression of shRNA-expressing DNA
- Cell transformation (recombinant protein-producing cells, immortalized cells, disease model cell establishment, etc.)
- Reporter assays, etc.
Transient and stable expression
There are two main ways to put genes into cells and make them work, as shown in the figure above.
One method is called transient gene expression. In this method, the introduced gene usually does not become part of the cell’s own DNA. Because of this, the gene effect becomes weaker as the cells continue to divide. This method is useful for studies that only need short-term gene activity, such as reporter assays or temporary gene suppression tests.
The other method is called stable gene expression. In this method, the introduced gene becomes part of the cell’s DNA. Because of this, the gene can continue to work even after many rounds of cell division. This method is useful for making cell lines that continuously produce a target gene over a long period.
We can provide both transient and stable gene expression systems depending on the purpose of your study.
*Studies will be mainly conducted by those with doctoral degrees.
