Study Overview
NADPH oxidase (NOX) is a membrane-bound enzyme complex that produces reactive oxygen species (superoxide radicals). This enzyme is used in neutrophils for bactericidal purposes. However, excessive activation can cause oxidative stress, which is one of the causes of arteriosclerosis, so its activity must be appropriately controlled.
This study evaluates the effect of the test substance on NOX activity. Because NOX activity measurement requires the enzyme complex, a crude cell extract fraction is used as the enzyme solution. Homogenization also makes it possible to measure NOX activity in tissues.
Study example
NOX activity using NADPH oxidase extracted from HUVEC
It is significantly activated by the activator, and its activity is suppressed by adding SOD, a superoxide scavenging enzyme.
Basic Experimental design
| Cells used | None (in vitro test) (Crude NOX active fractions were extracted from normal human umbilical vein endothelial cells (HUVEC) and human myeloid leukemia cells (HL-60) and used as enzyme solutions.) |
|---|---|
| Measurement items | NOX activity measurement |
*Studies will be mainly conducted by those with doctoral degrees.
