Study Overview
Arteriosclerosis (atherosclerosis) is a pathological condition caused by lifestyle-related factors and is strongly correlated with various other illnesses. The process of arteriosclerosis begins when cholesterol, including oxidized cholesterol (LDL), enters the vascular intima from the blood. Immune cells in the vascular intima, such as macrophages and T cells, recognize the LDL as a foreign substance and ingest it, releasing cytokines and other substances. These cytokines and other substances stimulate smooth muscle cells, causing them to infiltrate and migrate into the vascular intima. This thickens the arterial wall, leading to the impairment blood flow and increased risk of blood clot formation. Substances that are able to prevent or improve atherosclerosis are vital for preventing disorders such as strokes and heart attacks.
In this study, an arteriosclerotic model is used to evaluate if a test substance has anti-atherosclerotic effects. This model is created using human aortic smooth muscle cells.
Basic Experimental design
| Cells used | Human aortic smooth muscle cells (AoSMC), etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | Cell proliferation inhibition, Cell count of invading cells |
*All our studies are mainly performed and handled by our team of PhD scientists.
