Study Overview
Apoptosis, also known as programmed cell death, is a phenomenon that occurs in various cells with the aim of maintaining homeostasis in the body. In the field of oncology, apoptosis induction is often used as one of the indicators for evaluating the effectiveness of therapeutic drugs, and compounds that have the ability to induce apoptosis in cancer cells have the potential to become promising anticancer drugs.
This study will examine the apoptosis-inducing effects of test substances using various cell types, with the aim of verifying and discovering new functional substances that induce apoptosis.
Study example
Fluorescence microscopy allows visual assessment of whether a test substance is inducing apoptosis.
・Early apoptosis detection: Annexin V (green)
・Late apoptosis (dead cells): PI (red)
・Nuclei (total cell count): Hoechst (blue)
Test substances A and C showed early induction of apoptosis.
Test articles A and C also resulted in cell death.
Test substances A and C reduced the total cell count.
Basic Experimental design
| Cells used | Various cells |
|---|---|
| Measurement items | Annexin V, Propidium Iodide (PI), Hoechst33342, etc. |
| Main study | Imaging using a fluorescence microscope. Measurement using a flow cytometer, plate reader, and image analysis. |
*Studies will be mainly conducted by those with doctoral degrees.
