Study Overview
Bone structure and strength are maintained through a balance between bone formation by osteoblasts and bone resorption by osteoclasts.
This study will examine the effects of test substances on osteoblast and osteoclast differentiation, with the aim of verifying functional substances that support bone health and searching for new substances.
Mouse osteoblastic cell line (MC3T3-E1)
Osteoclasts differentiated from rat bone marrow cells
(TRAP staining image)
Study example
Basic Experimental design
| Cells used | Primary osteoblasts, MC3T3-E1, etc. Primary osteoclast precursor cells, Raw264.7, etc. |
|---|---|
| Preliminary study | Cytotoxicity assay |
| Main study | <Studies using osteoblasts> ・Cell activation study ・ALP activity measurement ・Osteocalcin level measurement ・Alizarin red staining, etc. ・Cell activation test ・ALP activity measurement ・Osteocalcin level measurement ・Alizarin red staining, etc. ・Cell activation test ・TRAP staining, etc. |
*Studies will be mainly conducted by those with doctoral degrees.
